产品简介: 该产品是一款用于SDS-PAGE蛋白电泳的浓缩缓冲液,稀释后的工作液能 极大提高蛋白电泳速率,大幅缩短电泳时间,在199V-251V电压,31min左右 即可完成电泳过程,且电泳分离后的蛋白条带细窄锐利。 该产品为20×浓缩液,使用前按照比例加纯水稀释至1×即可适用于普通变 性聚丙烯酰胺凝胶体系。.取25ml浓缩液加入475ml纯水混匀,得到500ml的1×电泳工作液。
Product Description
This product is a concentrated running buffer designed for SDS-PAGE protein electrophoresis. When diluted to working concentration, it drastically accelerates protein migration and significantly shortens electrophoresis runtime. The entire separation process can be completed in approximately 31 minutes under a voltage range of 199 V to 251 V, and it yields thin, sharp, well-resolved protein bands after electrophoresis.
Supplied as a 20× concentrated stock solution, this buffer can be diluted with pure water to a 1× working concentration prior to use, compatible with standard denaturing polyacrylamide gel systems.
Preparation protocol for 500 mL of 1× running buffer: Mix 25 mL of the 20× concentrated buffer with 475 mL of pure water and homogenize thoroughly.